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Award details
Folding and assembly of bacterial enterotoxins: analysis of the roles of periplasmic folding factors
Reference
C10318
Principal Investigator / Supervisor
Professor Robert Freedman
Co-Investigators /
Co-Supervisors
Dr Lloyd Ruddock
Institution
University of Kent
Department
Sch of Biosciences
Funding type
Research
Value (£)
250,494
Status
Completed
Type
Research Grant
Start date
01/03/1999
End date
01/09/2002
Duration
42 months
Abstract
The project will build on our current work on the stability and disassembly of txB, and on catalysts of native disulphide bond formation. It will be integrated into continuing programmes on catalysis of protein folding and on toxin biosynthesis. a) We will study the refolding and re-assembly of P93G and P93A mutants of txB and wild-type subunits, both disulphide-bonded and from the reduced state, in absence and presence of A subunits or peptide fragments of A subunit. b) We will study in detail the effects of periplasmic folding factors, DsbA and PPI, on this refolding and reassembly in vitro. c) We will use this system both to characterise mutants of DsbA and is isolate and define the structures and dynamics of DsbA-bound intermediates.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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