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Mapping of an interaction required for assembly of the anaphase promoting complex
Reference
C10298
Principal Investigator / Supervisor
Dr Johanna Avis
Co-Investigators /
Co-Supervisors
Institution
The University of Manchester
Department
Life Sciences
Funding type
Research
Value (£)
175,484
Status
Completed
Type
Research Grant
Start date
01/09/1998
End date
01/09/2001
Duration
36 months
Abstract
Targeting of cyclin B for ubiquination at mitosis is achieved by the anaphase promoting complex (APC), a multi-subunit ubiquitin protein ligase of which three components; Cdc16- p, Cdc23p and Cdc27p possess multiple tetratricopeptide repeat (TPR) motifs. The primary goal of this proposal is to map the TPR-mediated interaction between Cdc27p and Cdc23p. The work will be extended to investigate interactions of these proteins with further APC components and APC substrates. We will work towards the obtaining preliminary structural data on a complex between interacting domains of Cdc23p and Cdc27p. Results will have implications for the APC assembly pathway and for protein recognition of the TPR motif. A clearer view of APC structure and function could ultimately lead to the development of novel anti-cancer therapeutic compounds.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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