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Mechanistic mapping and redox regulation in NO- synthase and a related monooxygenase
Reference
C10203
Principal Investigator / Supervisor
Professor Stephen Chapman
Co-Investigators /
Co-Supervisors
Institution
University of Edinburgh
Department
Sch of Chemistry
Funding type
Research
Value (£)
182,153
Status
Completed
Type
Research Grant
Start date
01/10/1998
End date
31/12/2001
Duration
38 months
Abstract
The nitric oxide synthases are eukaryotic P450-like flavocytochromes which catalyse the production of citrulline and nitric oxide (NO) from arginine. They are important enzymes because of the roles of NO in cellular signalling processes. We aim to investigate the way in which the redox properties of neuronal nitric oxide synthase (nNOS) are regulated. This will be achieved by determining the underlying thermodynamic and kinetic properties of the enzyme. In addition we shall exploit the similarities between nNOS and its closest prokaryotic relative, flavocytochrome P450 BM3 to further examine the distinct mechanisms by which electron transfer is controlled. This will be done using chimeras between nNOS and P450 BM designed to switch the redox control mechanisms in these enzymes.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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