BBSRC Portfolio Analyser
Award details
Examination of endoplasmic reticulum-associated degradation (ERAD) in vitro
Reference
C09893
Principal Investigator / Supervisor
Dr Mark Field
Co-Investigators /
Co-Supervisors
Institution
Imperial College London
Department
Biological Sciences
Funding type
Research
Value (£)
202,832
Status
Completed
Type
Research Grant
Start date
01/10/1998
End date
01/10/2001
Duration
36 months
Abstract
We propose a new approach to examine ERAD in a novel in vitro system and will exploit observations in our laboratory that failure to remove a glycosylphosphatidylinositol membrane anchor signal sequence leads to retention and degradation of the protein by ERAD. The studies will be based on a microsome system for in vitro import into the ER and will be programmed with an engineered protein, preprominiPLAP, based on placental alkaline phosphatase with a defective GPI signal. We will have two major aims; firstly to demonstrate re-export of the protein from the ER and, secondly, to characterise factors involved both in recognition and delivery of ERAD substrates to the export system and those involved in extraction from the ER and delivery to the proteasome
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search