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Real time analysis of the trafficking of TRH receptor splice variants and mutants using green fluorescent protein chimeras

Reference	C09891
Principal Investigator / Supervisor	Professor Graeme Milligan
Co-Investigators / Co-Supervisors	Professor Gwyn William Gould
Institution	University of Glasgow
Department	IBLS Division of Biochemistry & Molecula
Funding type	Research
Value (£)	197,961
Status	Completed
Type	Research Grant
Start date	01/05/1999
End date	17/10/2002
Duration	41 months

Abstract

The dynamic internalisation and recycling of G protein-coupled receptors (GPCRs) is enhanced by agonist ligands and is intimately involved in the desensitisation and resensitisation of cellular response. Using green and blue fluorescent protein-linked constructs of the long and short isoforms of the thyrotrophin releasing hormone receptor, and mutations of these receptors which are know or are likely to regulate their degree of internalisation and constitutive activity, we will provide a detailed molecular description of the trafficking pathways of GFP-tagged GPCRs, real time information on the mechanics of receptor movement in intact cells and an analysis of the contributions of signalling pathways to these processes.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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