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Protein 4.1 in neurons: expression in neuronal differentiation activities and regulation
Reference
C09629
Principal Investigator / Supervisor
Dr Anthony Baines
Co-Investigators /
Co-Supervisors
Institution
University of Kent
Department
Sch of Biosciences
Funding type
Research
Value (£)
175,075
Status
Completed
Type
Research Grant
Start date
01/09/1998
End date
01/09/2001
Duration
36 months
Abstract
Protein 4.1 (p4.1) is a component of the plasma membrane-associated cytoskeleton in most animal cells. Despite being the subject of investigation in erythrocytes for many years, its role in complex tissue is still poorly understood. This project will investigate the role and regulation of p4.1 in neurons, using the P19 cell line as a model. P19 mouse embryonal carcinoma cells can be differentiated to neurons, accompanied by changes in p4.1 isotype expression. To determine the nature and roles of individual p4.1 isotypes, their mRNAs will be characterised and their expression inhibited by anti-sense oligonucleotides. High molecular mass isotypes of p4.1 are rapidly induced at synaptogenesis, and are components of post-synaptic densities. p4.1 interactions with PSD proteins in vitro will be analysed. A 65 kDa isoform of p4.1 which interacts only weakly with the cytoskeleton will be investigated.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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