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Award details
Dissection of the enzyme activities involved in transcription and replication of the dsRNA genome of bluetongue virus
Reference
C09624
Principal Investigator / Supervisor
Professor Polly Roy
Co-Investigators /
Co-Supervisors
Institution
London Sch of Hygiene and Trop Medicine
Department
Infectious and Tropical Diseases
Funding type
Research
Value (£)
185,684
Status
Completed
Type
Research Grant
Start date
15/09/1998
End date
31/03/2002
Duration
42 months
Abstract
The purpose of this project is to precisely describe the enzymatic processes required for replication and mRNA transcription of a model double-stranded RNA virus, BTV. The work is relevant to the equivalent processes that occur in a number of related viral pathogens (avian, livestock; reoviruses, rotaviruses, orbiviruses). BTV is well studied at both the structural and molecular level but still lacks a complete description of its replication biochemistry. The study will utilise novel assay systems already developed and highly purified baculovirus-expressed BTV proteins and exact-copy RNA transcripts of genomic segments as templates. Reconstituted subviral particles containing specific combinations of the enzymatic components will also be used. These reagents will allow the identity and dissection of the component activities and, by site-directed mutagenesis, the location of the enzymatic active sites.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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