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Regulation of the secretory machinery in living plant cells
Reference
C09622
Principal Investigator / Supervisor
Professor Christopher Hawes
Co-Investigators /
Co-Supervisors
Professor David Evans
Institution
Oxford Brookes University
Department
Faculty of Health and Life Sciences
Funding type
Research
Value (£)
130,924
Status
Completed
Type
Research Grant
Start date
30/06/1998
End date
30/06/2001
Duration
36 months
Abstract
Tobacco plants will be transformed with green fluorescent protein constructs targeted to either the endoplasmic reticulum (ER), Golgi apparatus or both ER and Golgi. Regulatory and structural proteins with putative roles in ER-Golgi transport will be heterologously expressed and purified. Microinjection techniques will be used to introduce mutant recombinant proteins, antipeptide antibodies and antisense nucleic acids into leaf cells and trichomes to investigate structural effects on components of the secretory pathway and the transport of product and membrane through the pathway. Data will be collected by confocal microscoy (real time and time lapse) plus electron microscopy of injected cells.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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