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Regulation of SpoIIE the phosphatase needed to establish differential gene expression in sporulating B. subtilis
Reference
C09490
Principal Investigator / Supervisor
Dr M Yudkin
Co-Investigators /
Co-Supervisors
Institution
University of Oxford
Department
Biochemistry
Funding type
Research
Value (£)
160,015
Status
Completed
Type
Research Grant
Start date
01/11/1998
End date
30/11/2002
Duration
49 months
Abstract
Initiation of differential gene expression in Bacillus subtilis depends on a four- component switch, of which the central regulator is the protein phosphatase SpoIIE. How SpoIIE is itself regulated is unknown. We shall use several techniques to look for cellular proteins that interact with SpoIIE and either regulate its activity or degrade it. Candidate proteins will be purified and micro-sequenced, and their genes will be identified and cloned. After confirming the regulatory role of these genes with knockout mutations, we shall determine the timing of their expression and the cellular location of their products. We shall also over-express and purify the proteins and thus study their interactions with SpoIIE in detail.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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