BBSRC Portfolio Analyser
Award details
Molecular mechanisms of gating of G-protein- activated inwardly rectifying K channels
Reference
C09333
Principal Investigator / Supervisor
Dr Ruth Murrell-Lagnado
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Pharmacology
Funding type
Research
Value (£)
179,304
Status
Completed
Type
Research Grant
Start date
17/08/1998
End date
31/12/2001
Duration
40 months
Abstract
G protein-activated inward rectifier K+ channels (GIRKs) are found in central neurones where they act to suppress firing. They couple to receptors via pertussis toxin-sensitive G-proteins and are directly activated by the binding of G beta g subunits. They have been cloned and shown to have 2 transmembrane regions. The native neuronal channels are heteromers composed of GIRK1 and GIRK2. We have made a series of chimeric subunits composed of GIRK1 and GIRK2 and these differ in their voltage-dependent gating properties and also in their gating by internal ligands. The aim of this proposal is to understand the molecular mechanisms of gating of recombinant GIRKs expressed in Xenopus oocytes using electrophysiological and molecular biological methods.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search