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Manipulating frost sensitivity and the basis of acyl specificity of acyltransferases
Reference
C09288
Principal Investigator / Supervisor
Professor David Rice
Co-Investigators /
Co-Supervisors
Institution
University of Sheffield
Department
Molecular Biology and Biotechnology
Funding type
Research
Value (£)
173,554
Status
Completed
Type
Research Grant
Start date
01/07/1998
End date
01/07/2001
Duration
36 months
Abstract
The synthesis of lipids containing differential levels of cis unsaturated versus saturated fatty acids lies at the heart of the phenomena of chilling resistance in plants. Diffraction quality crystals of Squash Glycerol-3-phosphate-1-acyltransferase (G-3-P-AT), the enzyme which catalyses the incorporation of the acyl group from acyl-Acyl Carrier Protein or acyl CoA's into glycerol-3-phosphate, has been obtained and this proposal aims to determine its three dimensional structure. Once completed the structure will be analysed against the sequence of the highly homologous Arabidopsis enzyme to provide new insights into the molecular basis of its specificity for cis-unsaturated fatty acids versus saturated fatty acids and to enhance our understanding of factors influencing substrate specificity and thereby frost sensitivity.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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