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Characterisation of key events involved in the self-incompatibility (SI) response in Papaver rhoeas

Reference	C09221
Principal Investigator / Supervisor	Professor Vernonica E. (Noni) Franklin-Tong
Co-Investigators / Co-Supervisors	Professor Chris Franklin
Institution	University of Birmingham
Department	Sch of Biosciences
Funding type	Research
Value (£)	182,107
Status	Completed
Type	Research Grant
Start date	01/01/1998
End date	01/01/2001
Duration	36 months

Abstract

Studies of the molecular and biochemical basis of SI in P. rhoeas provides an excellent system in which to investigate many aspects of intracellular signalling. Here we propose to investigate the downstream events triggered by the Ca2+ -dependent signalling pathway. This will include [A] Analysis of the cellular responses to the SI response and identification of the timing and mechanisms involved in irreversible pollen tube growth and cell death. Although we have evidence that other events follow the Ca2+ signal, whether this is a passive process or an active process (e.g. apoptosis) is not known. However, given the involvement of Ca2+ signalling, protein phosphorylation and gene transcription, the most likely scenario is that it is an active process. We will examine possible processes which may be involved, such as an oxidative burst and/or programmed cell death. [B] Cloning and characterisation of the genes expressed as a consequence of the SI response. This will give us a very good idea of the mechanisms involved in mediating the SI response.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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