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Cell-type specific monitoring of intracellular calcium in a Drosophila epithelium using an aequorin transgene

Reference	C08690
Principal Investigator / Supervisor	Professor Julian Dow
Co-Investigators / Co-Supervisors	Professor Shireen Davies
Institution	University of Glasgow
Department	Molecular Genetics
Funding type	Research
Value (£)	256,470
Status	Completed
Type	Research Grant
Start date	26/11/1997
End date	26/11/2000
Duration	36 months

Abstract

We have developed a new model for integrative epithelial physiology, that can be studied with the genetic power unique to Drosophila. The Malpighian tubule is amenable to analysis at the physiological, biochemical and molecular levels; and the underlying organisation of the tissue has been deduced genetically with P{GAL4} enhancer traps. Recently, we have successfully directed aequorin transgene expression to subsets of cells in the intact organism. We will now use this technology to study non-invasively the integration of hormonal signals within the genetic domains we have identified in this tissue. In addition to providing important data on this valuable insect model, we will be able to develop this generic technology for studies of cell signalling in Drosophila, and so expand the range of physiological phenotypes available for reverse genetic analysis.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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