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Identification of genes involved in the repair of a damaged membrane protein complex
Reference
C08665
Principal Investigator / Supervisor
Professor Peter Nixon
Co-Investigators /
Co-Supervisors
Institution
Imperial College London
Department
Biological Sciences
Funding type
Research
Value (£)
173,863
Status
Completed
Type
Research Grant
Start date
15/01/1998
End date
15/01/2001
Duration
36 months
Abstract
A mutagenesis approach using the green alga Chlamydomonas reinhardtii will be used to identify the gene products involved in the D1 repair cycle which is used to selectively replace a damaged D1 polypeptide within the multisubunit photosystem two complex. A library of insertionally inactivated nuclear mutants will be generated by complementing a nitrate reductase mutant (nit1) with the cloned nitrate reductase gene (NIT1). Screens involving the use of a fluorescence video imaging system and the sensitivity of growth to high light will be used as a first step to identify mutants within this library that are affected in (i) D1 synthesis (ii) the assembly on D1 into a photosystem two complex and (iii) the turnover of D1 in response to light conditions that promote damage to D1. The NIT1 gene will then be used as a tag to help clone the genes involved in the D1 repair cycle.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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