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The function of the extracellular domain of CD45: an in vivo genetic complementation study

Reference	C08628
Principal Investigator / Supervisor	Dr Nicholas Holmes
Co-Investigators / Co-Supervisors
Institution	University of Cambridge
Department	Pathology
Funding type	Research
Value (£)	346,532
Status	Completed
Type	Research Grant
Start date	01/11/1997
End date	31/12/2001
Duration	48 months

Abstract

CD45 is a transmembrane protein tyrosine phosphatase. We have generated a CD45-null knockout mouse line, analysis of which shows that CD45 is required for signal coupling in T cell development and in B cell maturation. CD45 exists as several isoforms which differ only in their extracellular (EC) domains. The expression of different isoforms is highly regulated during lymphocyte development and is conserved between species. The aim of this project is to use genetic complementation of the CD45 KO line to generate new transgenic mice expressing normal single isoforms or a mutant CD45 molecule lacking the entire EC domain. This will be achieved by insertional transgenesis. The results will elucidate the functional role of the CD45 EC domain.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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