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Regulation of cellular respiration by nitric oxide endothelial agonists and cytokines
Reference
C08510
Principal Investigator / Supervisor
Professor Guy Brown
Co-Investigators /
Co-Supervisors
Professor Martin Brand
,
Professor Salvador Moncada
Institution
University of Cambridge
Department
Biochemistry
Funding type
Research
Value (£)
156,685
Status
Completed
Type
Research Grant
Start date
01/02/1998
End date
01/02/2001
Duration
36 months
Abstract
We and others have shown that physiological levels of nitric oxide (NO) reversibly inhibit mitochondrial respiration at cytochrome oxidase, and that cells stimulated by cytokines to express iNOS regulate cellular respiration by this means. This project aims to determine whether NO is a physiological or pathophysiological regulator of cellular respiration and its affinity for oxygen, by testing whether and in what conditions endothelial agonists or cytokines causing NO release from ecNOS or iNOS in cultured endothelial cells, cultured vascular smooth muscle cells or isolated aorta can regulate the cellular respiration of these and surrounding cells via NO inhibition of cytochrome oxidase or peroxynitrite inhibition of the respiratory chain. Whether the known effects of NO and cytokines on cell growth, division, and death are mediated via the inhibition of respiration will also be tested.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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