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Regulation and activity of the mitotic polo-like kinases (plks) of Xenopus and Drosophila
Reference
C08491
Principal Investigator / Supervisor
Professor David Glover
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Genetics
Funding type
Research
Value (£)
209,785
Status
Completed
Type
Research Grant
Start date
01/02/1998
End date
31/01/2002
Duration
48 months
Abstract
We will determine the timing of activation of plx1 in Xenopus cell free systems to ascertain whether it could function to activate cdc25. Biochemical studies of Plx1 activity on Xenopus oocytes, and phenotypic studies of an allelic series of polo mutants in Drosophila will be used to study the function of the enzyme in meiosis. A combination of biochemical studies and in vitro mutagenesis will be used to determine phosphorylation sites on plks and their role in regulating activity. Finally, the effects of plk-mediated phosphorylation will be determined from the function of a number of microtubule associated proteins including kinesin-like proteins. This will be related to studies on the effects of plks upon the behaviour of centrosomes in microtubule nucleation arrays and assays for spindle formation in vitro.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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