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The thermodynamic and kinetic profile of flavocytochrome P-450 BM3
Reference
C08422
Principal Investigator / Supervisor
Professor Stephen Chapman
Co-Investigators /
Co-Supervisors
Institution
University of Edinburgh
Department
Sch of Chemistry
Funding type
Research
Value (£)
159,521
Status
Completed
Type
Research Grant
Start date
01/10/1997
End date
01/10/2000
Duration
36 months
Abstract
Flavocytochrome P-450 BM3 is an extremely important enzyme. This enzyme from Bacillus megaterium is the only soluble P-450 to use a mammalian-like diflavin P-450-reductase. In addition, the reductase domain is covalently linked to the P-450 haem domain providing a one-component system for study. We aim to provide an integrated thermodynamic and kinetic profile of this important enzyme by completing the following objectives: Determining the reduction potentials for all three cofactors in the enzyme (FAD, FMN and Haem); performing a thorough kinetic analysis of each electron transfer step in the enzyme; and mutating key residues to determine their effects on the redox characteristics of the enzyme.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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