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The mechanism of action of acidic transcriptional activators

Reference	C08418
Principal Investigator / Supervisor	Dr Stefan Roberts
Co-Investigators / Co-Supervisors
Institution	University of Dundee
Department	College of Life Sciences
Funding type	Research
Value (£)	140,218
Status	Completed
Type	Research Grant
Start date	16/02/1998
End date	16/02/2001
Duration	36 months

Abstract

Transcription of a protein-coding gene requires the assembly at the promoter of the general transcription factors and RNA polymerase II. Transcriptional activator proteins stimulate this process, although the mechanisms involved are unclear. VP16 of Herpes virus is a model activator that is used extensively in the transcription field to study fundamental mechanisms of eukaryotic transactivator function. Chemical crosslinking will be used to analyse the interaction between VP16 and a central component of the transcription machinery, TFIIB. Strategic mutagenesis of TFIIB will be performed to provide insights in to the nature and consequences of the VP16-TFIIB interaction. We have identified a novel TFIIB-interacting factor that is required for transcriptional activation and this will be cloned and studied further. Finally, in vivo analyses of activator-TFIIB contacts will be performed.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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