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Award details
Purification and characterisation of Holliday junction resolving enzymes from archaea
Reference
C08300
Principal Investigator / Supervisor
Prof. Malcolm White
Co-Investigators /
Co-Supervisors
Institution
University of St Andrews
Department
Biology
Funding type
Research
Value (£)
160,625
Status
Completed
Type
Research Grant
Start date
01/02/1998
End date
01/02/2001
Duration
36 months
Abstract
The process of homologous recombination involves the creation and manipulation of Holliday junctions and the rearrangement of DNA, which is essential for the generation of genetic diversity. The enzymology of homologous recombination is poorly understood outwith the eubacteria. The aim of this project is to purify junction specific endonucleases from archaea. Purified enzymes will be partially sequenced and the relevant genes cloned and over-expressed. A battery of biophysical techniques will be employed to characterise the enzymes and the nature of their interaction with the DNA junction. These studies will shed light on the recombination pathway of the archaea and possibly the eucarya, and on the relationship between the two kingdoms.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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