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Functional analysis of gating in TOKI and related K+ channels
Reference
C07797
Principal Investigator / Supervisor
Professor Michael Blatt
Co-Investigators /
Co-Supervisors
Professor Simon Jarvis
Institution
Imperial College London
Department
Biological Sciences
Funding type
Research
Value (£)
170,827
Status
Completed
Type
Research Grant
Start date
15/03/1997
End date
15/03/2000
Duration
36 months
Abstract
Gating of TOKI, a K+ channel cloned from Saccharomyces, is a model for outward- rectifying K+ channels in plants and fungi that are sensitive to extracellular [K+] as well as voltage. The effect is analogous to animal anomalous rectifiers, but the mechanisms clearly differ. TOKI includes an unusual double-pore structure and lacks the archetypal membrane-spanning voltage sensor. We have begun site-directed mutagenesis, expressing TOKI in Xenopus oocytes, and find that the gate is regulated by K+ binding at a site removed from the channel permeation pathway. Funds are requested to continue this research (1) to characterise the amino acid sequences of TOKI that contribute to the extracellular K+- and voltage-sensitivities of gating and (2) to use a TOKI deletion to screen for other outward-rectifying K+ channels by complementation in yeast.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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