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Signal transduction coupling photoreception by CRY1 to CHS transcription in Arabidopsis
Reference
C07777
Principal Investigator / Supervisor
Professor Gareth Islwyn Jenkins
Co-Investigators /
Co-Supervisors
Professor Marc Knight
Institution
University of Glasgow
Department
IBLS Division of Biochemistry & Molecula
Funding type
Research
Value (£)
185,530
Status
Completed
Type
Research Grant
Start date
30/09/1997
End date
29/10/2003
Duration
73 months
Abstract
We will: 1. Investigate directly the involvement of particular cellular calcium pools in signal transduction initiated by UV-A/blue light absorption by the Arabidopsis CRY1 photoreceptor, using an Arabidopsis cell culture expressing targeted aequorin fusions. 2. Relate calcium signalling to the CRY1 photoreceptor, and to transcription of the Arabidopsis chalcone synthase gene (CHS), using selected aequorin fusions in a CRY1 -deficient hy4 mutant cell culture and transformation of the cells with the HY4 (CRY1) gene. 3. Investigate the cellular location of CRY1 using an epitope-tagged fusion. 4. Investigate the function of the C-terminal domain of CRY1 in initiating calcium signalling and CHS expression by introducing mutant HY4 fusions into hy4 cells.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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