Award details

Using protein ligands and site-directed mutants to study porin channel dynamics

Reference	C07652
Principal Investigator / Supervisor	Professor Jeremy Lakey
Co-Investigators / Co-Supervisors
Institution	Newcastle University
Department	School of Cell and Molecular Biosciences
Funding type	Research
Value (£)	167,507
Status	Completed
Type	Research Grant
Start date	01/11/1997
End date	11/07/2001
Duration	45 months

Abstract

Porins, by virtue of their channel size, stability, molecular biology and known high resolution structures offer a unique opportunity to study membrane channel dynamics. Recently they have been shown to be involved in protein translocation across membranes and to be ligand gated channels. This project aims to study several porin- ligand complexes by single-channel electrophysiological measurements, microcalorimetry, mutagenesis, 2D crystallography and MAS solid state NMR. Of particular interest are porin binding proteins from phage and recombinant expression systems for these will be developed. Understanding of the ligand gating will allow further development of porins as a generic biosensor.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search