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Fluorescent imaging of cGMP in cerebellar Purkinje cells during the induction of cerebellar long-term depression

Reference	C07457
Principal Investigator / Supervisor	Professor Nicholas Hartell
Co-Investigators / Co-Supervisors	Professor D Billington, Dr David Poyner
Institution	Aston University
Department	Sch of Life and Health Sciences
Funding type	Research
Value (£)	188,045
Status	Completed
Type	Research Grant
Start date	13/10/1997
End date	01/06/2001
Duration	44 months

Abstract

Here we propose to refine and extend a novel fluorescent imaging technique to investigate the synaptic inputs responsible for NO generation and cGMP production in cerebellar Purkinje cells. We will combine this technique with electrophysiological recording in brain slices to study the interaction of calcium and cGMP in the process of long-term depression, a form of synaptic plasticity considered to underpin learning and memory in the cerebellum. We will also develop membrane permeable, fluorescent, cGMP analogues to facilitate measurements of cGMP levels in cells already containing the phosphodiesterase isoform, present in Purkinje cells and which is required for this technique. By isolating and co-injecting this enzyme with our cGMP measuring probe, we will extend the scope of this technique to a range of other cell types.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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