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A biochemical analysis of ribosomal frameshifting
Reference
C07089
Principal Investigator / Supervisor
Professor Ian Brierley
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Pathology
Funding type
Research
Value (£)
178,512
Status
Completed
Type
Research Grant
Start date
01/01/1997
End date
01/01/2000
Duration
36 months
Abstract
This project will investigate the process of ribosomal frameshifting on messenger RNAs. The focus will be on how the two components of the frameshift signal, the `slippery' sequence and RNA pseudoknot structure interact with the cellullar translation machinery. We will employ a variety of methods, including tRNA depletion, in vitro translation, RNA structure mapping, site-specific crosslinking and ribosomal pausing assays to address three questions: 1) the role in frameshifting of tRNA anticodon modification, both in vitro and in vivo; 2) how RNA pseudoknot structure and function are interrelated, and 3) the identity of the ribosomal proteins and rRNA sequences which interact with RNA pseudoknots during translation.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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