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Interactions of inositol trisphosphate receptors with their ligands analysed by scintillation proximity assay
Reference
C07039
Principal Investigator / Supervisor
Professor Colin Taylor
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Pharmacology
Funding type
Research
Value (£)
189,190
Status
Completed
Type
Research Grant
Start date
01/01/1997
End date
01/01/2000
Duration
36 months
Abstract
SPA, which we have already applied to characterise IP3 binding to purified IP3 receptors, will be combined with stop-quench methods to examine the rapid kinetics of IP3 binding. Antipeptide antibodies will be used to attach IP3 receptor subtypes to SPA beads to allow their binding characteristics to be determined. The binding of small messengers (ATP, NADH, Ca2+), pharmacological agents (e.g. fura 2) and proteins (e.g. calmodulin) to pure IP3 receptors will be characterised and relevant interactions between them examined. We aim also to assess the practicability of applying SPA methods to other aspects of Ca2+ signalling pathways: the effects of Ca2+ antagonists on 5-HT3 receptors, the interactions of ligands with ryanodine receptors, and receptor-G-protein interactions.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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