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Purification and characterisation of a putative phosphatidylinositol 345-trisphosphate receptor
Reference
C07024
Principal Investigator / Supervisor
Professor Peter Cullen
Co-Investigators /
Co-Supervisors
Institution
University of Bristol
Department
Biochemistry
Funding type
Research
Value (£)
143,619
Status
Completed
Type
Research Grant
Start date
08/03/1997
End date
08/03/2000
Duration
36 months
Abstract
It is becoming increasingly apparent that the metabolism of the phosphoinositides catalysed by phosphoinositide (PI) 3-kinase has signal-transducing consequences that rival those of phosphoinositidase C activation in a variety of cells. Phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) is a major cellular product of agonist-activated PI 3-kinase and has therefore been proposed to act as a second messenger; thus suggesting the existence of an in vivo PtdIns(3,4,5)P3 receptor. It is towards purifying and characterising this putative PtdIns(3,4,5)P3 receptor that this proposal is aimed. Experimentally, it is proposed to use [32P]inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P4)-binding to follow purification, since chemically PtdIns(3,4,5)P3 has Ins(1,3,4,5)P4 as its polar headgroup.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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