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Constitutive activation of D2 dopamine receptors and the mechanism of inverse agonism

ReferenceC06913
Principal Investigator / Supervisor Professor Philip Gordon Strange
Co-Investigators /
Co-Supervisors
Institution University of Reading
DepartmentAnimal and Microbial Sciences
Funding typeResearch
Value (£) 119,232
StatusCompleted
TypeResearch Grant
Start date 07/01/1997
End date 07/01/2000
Duration36 months

Abstract

Coupling of D2 dopamine receptors to G-proteins in the absence of agonist stimulation (constitutive activation) and reversal of this constitutive activation by inverse agonists will be investigated in recombinant CHO cells expressing D2 receptors. Parameters affecting constitutive activation and inverse agonism will be examined including the effect of different levels of receptor expression, and structural requirements in the ligand and in the receptor. The mechanism of inverse agonism and the effect of stimulation, short and long term with inverse agonist, on receptor levels will be examined. Inverse agonism in native tissues expressing D2 receptors will be probed. Overall the results will provide exciting new information on receptor function.

Summary

unavailable
Committee Closed Committee - Biochemistry & Cell Biology (BCB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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