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Characterisation of the glyoxysomal protein import machinery by cross-linking of translocation intermediates
Reference
C06847
Principal Investigator / Supervisor
Professor Alison Baker
Co-Investigators /
Co-Supervisors
Institution
University of Leeds
Department
Plant Biochemistry & Biotechnology
Funding type
Research
Value (£)
154,122
Status
Completed
Type
Research Grant
Start date
06/01/1997
End date
06/01/2000
Duration
36 months
Abstract
Work from this laboratory using a glyoxysome in vitro protein import system has resulted in the discovery of two potential intermediates in protein translocation. A protein A-glycolate oxidase fusion protein forms an incompletely translocated transmembrane species in the presence of ATP. In the absence of ATP the fusion protein is surface-bound but can be chased to the transmembrane form upon addition of ATP. As the fusion protein can be imported in microgramme quantities and can be reisolated by virtue of its affinity for IgG, these two potential intermediates are excellent tools for cross-linking studies to identify nearby components of the glyoxysome import machinery.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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