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Selective agonist definition of G-protein channelling

Reference	C06724
Principal Investigator / Supervisor	Professor Graeme Milligan
Co-Investigators / Co-Supervisors
Institution	University of Glasgow
Department	IBLS Division of Biochemistry & Molecula
Funding type	Research
Value (£)	169,121
Status	Completed
Type	Research Grant
Start date	01/06/1997
End date	01/06/2000
Duration	36 months

Abstract

The issue of whether structurally distinct agonists for a single G-protein-coupled receptor can result in selective activation of different G-proteins (channelling) by promoting the stabilisation of different receptor conformations is one of the major current questions in receptor molecular pharmacology. We will address this question using stable cell lines co- transfected to express either the beta2-adrenoceptor or the delta-opioid receptor along with the universally promiscuous G-protein G16alpha. The ability of agonists at these receptors to selectively activate G16 compared to the endogenously expressed G-proteins normally associated with these receptors will be established. Transient expression studies using both wild type and pertussis toxin-resistant mutations of G1-like proteins will be used to ascertain whether this concept can be expanded to include closely related G-proteins.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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