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Interaction of yeast calmodulin with three proteins showing Ca2+-sensitive calmodulin binding
Reference
C06128
Principal Investigator / Supervisor
Professor Michael Stark
Co-Investigators /
Co-Supervisors
Institution
University of Dundee
Department
College of Life Sciences
Funding type
Research
Value (£)
151,596
Status
Completed
Type
Research Grant
Start date
15/08/1996
End date
15/08/1999
Duration
36 months
Abstract
The ubiquitous eukaryotic regulator calmodulin (CaM) mediates many cellular responses to elevated intracellular (Ca2+). My lab is investigating yeast CaM function, focusing to date on a critical CaM target protein at the spindle pole body. While CaM most often binds its targets in response to Ca2+, Ca2+-independent and Ca2+-sensitive binding also occur. Here it is proposed to characterise the Ca2+-sensitive CaM binding to 3 novel yeast targets, 2 of which are involved in vacuolar function. For each, CaM binding affinity and its Ca2+dependence will be examined and the site of CaM binding mapped by mutagenesis. Mutations which block CaM binding will be introduced into the yeast genome to determine the significance of CaM binding in vivo.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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