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Investigation of the influence of perturbations of the cAMP relay dynamics on multicellular Dictyostelium morphogenesis
Reference
C06124
Principal Investigator / Supervisor
Professor Kees Weijer
Co-Investigators /
Co-Supervisors
Institution
University of Dundee
Department
College of Life Sciences
Funding type
Research
Value (£)
209,411
Status
Completed
Type
Research Grant
Start date
01/07/1996
End date
01/07/1999
Duration
36 months
Abstract
We will test the effects of pertubations in the dynamics of the cAMP relay system on Dictyostelium morphogenesis. We will perturb the kinetics of the cAMP relay reaction by (1) using mutants defect in or expressing cAMP receptors of altered cAMP affinity. (2) By titrating the level of cAMP phosphodiesterase in synergy experiments between wild type and phosphodiesterase null cells. (3) By microinjection of cAMP pulses of variable duration, amplitude and frequency in slugs to interfere with endogenous cAMP signalling. We will measure wave propagation and cell movement in GFP expressing cells. Essential to the interpretation of the results and design of the experiments is the development of computer models for cAMP relay and cell movement during mound and slug formation.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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