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Architecture and function of the RNA polymerase II holoenzyme `Mediator' complex
Reference
C05917
Principal Investigator / Supervisor
Dr Robert Weinzierl
Co-Investigators /
Co-Supervisors
Institution
Imperial College London
Department
Biological Sciences
Funding type
Research
Value (£)
183,333
Status
Completed
Type
Research Grant
Start date
01/12/1996
End date
30/06/2000
Duration
42 months
Abstract
The recently discovered RNA polymerase II holoenzyme contains many of the components required for activated transcription. Functional studies imply that the mediator complex (SUG1, GAL11 and at least 9 SRBs) is involved in communicating with promoter-specific transcriptional activators. In this application I propose to produce recombinant versions of SUG1, GAL11 and of all known SRBs. These proteins will be used in a variety of protein:protein interaction assays to elucidate their topological arrangement within the mediator, their interaction with various transcriptional activators and other holoenzyme subunits. Attempts will be made to reconstitute the mediator in vitro to dissect the functional contributions of individual subunits.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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