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Xyloglucan endotransglycosylase (XET) isoenzymes: diversity of action on donor and acceptor substrates

Reference	C05850
Principal Investigator / Supervisor	Professor Stephen Fry
Co-Investigators / Co-Supervisors
Institution	University of Edinburgh
Department	Inst of Cell and Molecular Biology
Funding type	Research
Value (£)	135,754
Status	Completed
Type	Research Grant
Start date	01/09/1996
End date	01/09/1999
Duration	36 months

Abstract

XETs are a group of recently discovered enzymes that catalyse the endotransglycosylation of xyloglucans (major structural polysaccharides of the plant cell wall) and possibly regulate plant growth. Although several XET genes have been shown to differ in their expression patterns, very little is known about the functional differences between isoenzymes of XET in growing plant tissues. Working with 5 native and 2 transgenic isoenzymes of XET, we will characterise their catalytic differences, especially with respect to the molecular site at which they attack the donor substrate (mid-chain, or nearer the reducing or non-reducing end); affinity for various acceptor substrates differing in composition and size; affinity for fucosylated vs non-fucosylated donor substrates; and response to pH, cations, reducing agents, cellulose and low temperature.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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