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Single-molecule actin-myosin interactions determined with an optical tweezers transducer
Reference
C05278
Principal Investigator / Supervisor
Dr Justin Molloy
Co-Investigators /
Co-Supervisors
Professor John Sparrow
Institution
University of York
Department
Biology
Funding type
Research
Value (£)
180,712
Status
Completed
Type
Research Grant
Start date
01/03/1996
End date
01/03/1999
Duration
36 months
Abstract
We will use the picoNewton, nanometre optical tweezers mechanical transducer that we have developed to determine (1) rate constants and the distortion-dependence of various reactions in the actomyosin cross-bridge cycle, (2) the dependence on azimuthal position of the thin filament upon ability of myosin and its subfragment to bind to actin, (3) the role and importance of various amino acids in the binding site of actin on myosin using contractile protein mutants from Drosophila melanogaster. We will also (4) improve the stability and frequency response of our apparatus in order to determine if the rapid transitions between attached states predicted by Huxley and Simmons occur, and to test our interpretation of the magnitude of the elemental movement.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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