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Control of the palmitoylation status of signal transducing polypeptides as a regulator mechanism
Reference
C05228
Principal Investigator / Supervisor
Professor Graeme Milligan
Co-Investigators /
Co-Supervisors
Institution
University of Glasgow
Department
IBLS Division of Biochemistry & Molecula
Funding type
Research
Value (£)
153,227
Status
Completed
Type
Research Grant
Start date
01/01/1996
End date
01/01/1999
Duration
36 months
Abstract
The regulation and functional significance of the palmitoylation status of G-protein-linked receptors and G-proteins will be analysed in cell systems in which the maintained presence of an agonist results in downregulation of both the receptor and its associated G-protein. The dynamics of palmitoylation of these polypeptides, how this is controlled by receptor agonists and whether alteration in the palmitoylation status compromises the physical interactions between receptors and G-proteins will be assessed. Palmitoylation resistant mutants of both Gila and Gola will be further modified to eliminate sensitivity to pertussis toxin allowing the interactions of these G-proteins with receptors to be monitored directly even in the presence of endogenously expressed wild type pertussis toxin-sensitive G-proteins. There is considerable interest in the pharmaceutical industry in means to control the lapidation status of a variety of signal transducing polypeptides and the current studies will provide highly novel information on the significance of dynamic acylation.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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