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Molecular analysis of key events in the targeting and assembly of mammalian PDC and OGDC
Reference
C05142
Principal Investigator / Supervisor
Professor John Lindsay
Co-Investigators /
Co-Supervisors
Institution
University of Glasgow
Department
IBLS Division of Biochemistry & Molecula
Funding type
Research
Value (£)
176,294
Status
Completed
Type
Research Grant
Start date
01/07/1996
End date
31/07/2000
Duration
49 months
Abstract
The major study will examine the role(s) of the extended N-terminal presequences located on the cytoplasmic E2 precursors of human PDC and OGDC. The PCR-amplified presequences (and various deletion constructs) will be fused directly to the CAT reporter gene for transfection analysis of their effects on the efficiency of E2 targeting and assembly in COS cells. In addition, the sub-gene encoding the putative compact E3 binding domain, integrated into the E1 enzyme of OGDC, will be overexpressed in E. coli and the purified domain examined for its efficacy as a competitive inhibitor in preventing OGDC and PDC reconstitution, for analysis of its stoichiometry of interaction (and that of native E1) with E3 and for structural analysis by 2D- NMR.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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