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Site-directed mutagenesis of cofactor binding sites in photosystem I of Chlamydomonas reinhardtii

Reference	C05119
Principal Investigator / Supervisor	Professor Saul Purton
Co-Investigators / Co-Supervisors	Professor M Evans
Institution	University College London
Department	Genetics Evolution and Environment
Funding type	Research
Value (£)	189,342
Status	Completed
Type	Research Grant
Start date	15/04/1996
End date	14/04/1999
Duration	36 months

Abstract

The properties of the redox cofactors of the photosystem I complex are extensively modified by the protein environment. Site-directed mutagenesis offers the possibility of determining the role of the primary and secondary structure in the binding and functioning of these cofactors. We propose a detailed molecular investigation of the binding niche for the iron- sulphur centre FeSx, and the putative binding regions for the quinone A1, and the chlorophylls P700 and A0 in the green alga Chlamydomonas. We will employ conventional site-directed mutagenesis as well as techniques combining mutagenesis with functional selection. This latter strategy will allow the rapid generation of functional mutants modified in green gel and Western analysis, the redox and functional activity by spectroscopic techniques.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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