Award details

Role of folding enzymes and molecular chaperones in the translocation, folding and assembly of procollagen in the endoplasmic reticulum

Reference	C04146
Principal Investigator / Supervisor	Professor Neil Bulleid
Co-Investigators / Co-Supervisors
Institution	The University of Manchester
Department	Life Sciences
Funding type	Research
Value (£)	141,965
Status	Completed
Type	Research Grant
Start date	01/07/1995
End date	01/07/1998
Duration	36 months

Abstract

This project sets out to identify accessory proteins that interact with procollagen during folding and assembly within the endoplasmic reticulum. These proteins may have a direct role in catalysing protein folding, eg PDI, PPI, P4H, or may chaperone translocated molecules preventing non-specific interactions prior to assembly, eg BiP, calnexin, Hsp47. This will be achieved by reconstituting the folding pathway of this protein in a semi- permeabilised cell system. As this is an in vitro system the individual components can be manipulated, providing a means by which cellular processes can be studied under different conditions and by which reagents such as chemical cross-linkers can be added and directed to proteins within the ER.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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