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Development of G-protein switches to control endogenous and exogenous plant cell circuits
Reference
C04102
Principal Investigator / Supervisor
Professor Paul Anthony Millner
Co-Investigators /
Co-Supervisors
Institution
University of Leeds
Department
Inst of Molecular & Cellular Biology
Funding type
Research
Value (£)
79,106
Status
Completed
Type
Research Grant
Start date
03/07/1995
End date
03/07/1997
Duration
24 months
Abstract
Chimaeric neurokinin (NK2) receptors which couple to GPa1 or other G- proteins will be expressed in Arabidopsis. In parallel, wild type NK2 receptors will be co-expressed with GP alpha 1:Gq alpha chimaeras. Transgenic plants will be examined for functional receptors and G-proteins by NK2-ligand and GTP gamma S binding studies. Expression patterns of the proteins will be examined by immunofluorescence microscopy. Finally, the effects of NK2, agonists on plant development, PIC and PLA2 activities, ion channel function and internal ionic homeostasis will be examined by integrated biochemical, electrophysiological and ion imaging approaches. The work will resolve the in vivo function of GP alpha 1 and provide an approach for in vivo study of other plant G-proteins.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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