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Molecular and kinetic characterisation of a model cytochrome P450 system
Reference
C03127
Principal Investigator / Supervisor
Professor John Lindsay
Co-Investigators /
Co-Supervisors
Professor John Coggins
,
Professor C Fewson
Institution
University of Glasgow
Department
IBLS Division of Biochemistry & Molecula
Funding type
Research
Value (£)
152,848
Status
Completed
Type
Research Grant
Start date
01/02/1994
End date
30/09/1997
Duration
44 months
Abstract
1. To locate those amino acid residues governing specificity/selectivity for fatty acid and hydrocarbon substrates and those determining kinetic properties by site-directed mutagenesis of P450 BM3. 2. To prepare and characterise the functional sub-domains which comprise the reductase of P450 BM3 by cloning and overexpressing the sub-genes in E. coli. 3. To determine the microscopic rate constants for P450 BM3 and its constituent domains and sub-domains by stopped flow kinetics. 4. To attempt to crystallise P450 BM3 and its reductase domain.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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