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The molecular basis of phospholipase C regulation by lipids and G-proteins
Reference
C02556
Principal Investigator / Supervisor
Dr Stephen James
Co-Investigators /
Co-Supervisors
Professor Charles Downes
Institution
University of Dundee
Department
College of Life Sciences
Funding type
Research
Value (£)
126,484
Status
Completed
Type
Research Grant
Start date
01/12/1994
End date
01/12/1997
Duration
36 months
Abstract
Although phospholipase C (PLC)-mediated hydrolysis of PtdInsP2 stands at the heart of many cellular responses to external stimuli, practically nothing is known about the catalytic mechanisms of this enzyme family. This is partly due to the complex nature of the substrates embedded within a membrane bilayer, a situation which is difficult to mimic accurately in vitro. Aspects which are not known include whether more predominant membrane lipids have a role in PLC activation, how PLCs associate with aggregated substrates and mechanisms by which G proteins activate PLCs. This project will investigate these and other features of interfacial catalysis of G protein-activated PLC-B isoforms using a range of techniques applied to purified and recombinant PLCs and G protein subunits.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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