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The molecular basis of phospholipase C regulation by lipids and G-proteins

ReferenceC02556
Principal Investigator / Supervisor Dr Stephen James
Co-Investigators /
Co-Supervisors
Professor Charles Downes
Institution University of Dundee
DepartmentCollege of Life Sciences
Funding typeResearch
Value (£) 126,484
StatusCompleted
TypeResearch Grant
Start date 01/12/1994
End date 01/12/1997
Duration36 months

Abstract

Although phospholipase C (PLC)-mediated hydrolysis of PtdInsP2 stands at the heart of many cellular responses to external stimuli, practically nothing is known about the catalytic mechanisms of this enzyme family. This is partly due to the complex nature of the substrates embedded within a membrane bilayer, a situation which is difficult to mimic accurately in vitro. Aspects which are not known include whether more predominant membrane lipids have a role in PLC activation, how PLCs associate with aggregated substrates and mechanisms by which G proteins activate PLCs. This project will investigate these and other features of interfacial catalysis of G protein-activated PLC-B isoforms using a range of techniques applied to purified and recombinant PLCs and G protein subunits.

Summary

unavailable
Committee Closed Committee - Biochemistry & Cell Biology (BCB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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