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The molecular basis of phospholipase C regulation by lipids and G-proteins

Reference	C02556
Principal Investigator / Supervisor	Dr Stephen James
Co-Investigators / Co-Supervisors	Professor Charles Downes
Institution	University of Dundee
Department	College of Life Sciences
Funding type	Research
Value (£)	126,484
Status	Completed
Type	Research Grant
Start date	01/12/1994
End date	01/12/1997
Duration	36 months

Abstract

Although phospholipase C (PLC)-mediated hydrolysis of PtdInsP2 stands at the heart of many cellular responses to external stimuli, practically nothing is known about the catalytic mechanisms of this enzyme family. This is partly due to the complex nature of the substrates embedded within a membrane bilayer, a situation which is difficult to mimic accurately in vitro. Aspects which are not known include whether more predominant membrane lipids have a role in PLC activation, how PLCs associate with aggregated substrates and mechanisms by which G proteins activate PLCs. This project will investigate these and other features of interfacial catalysis of G protein-activated PLC-B isoforms using a range of techniques applied to purified and recombinant PLCs and G protein subunits.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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