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Stimulus-induced oscillations in guard cell cytosolic free Ca2+

Reference	C02365
Principal Investigator / Supervisor	Dr Martin Mcainsh
Co-Investigators / Co-Supervisors	Professor Alistair Hetherington
Institution	Lancaster University
Department	Biological Sciences
Funding type	Research
Value (£)	137,278
Status	Completed
Type	Research Grant
Start date	01/12/1994
End date	01/07/1998
Duration	43 months

Abstract

Fluorescence ratio photometry and imaging techniques will be used to study the kinetics, generation and maintenance of oscillations, and putative waves, in cytosolic free Ca2+ ([Ca2+]i) in stomatal guard cells in response to external Ca2+ ([Ca2+]e). The ability of two plant hormones, abscisic acid and auxin, and temperature to modulate oscillations in [Ca2+]i in response to [Ca2+]e will be investigated. The contribution of [Ca2+]e and intracellular Ca2+ stores to the oscillatory behaviour will be studied, and the signal transduction elements involved in the generation and maintenance of oscillations will be examined using caged probes and tobacco transformed with antisense constructs of known signal transduction genes.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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