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A Brassica cytokinin receptor
Reference
C01401
Principal Investigator / Supervisor
Dr David Ernest Hanke
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Plant Sciences
Funding type
Research
Value (£)
105,378
Status
Completed
Type
Research Grant
Start date
18/01/1994
End date
14/04/1997
Duration
39 months
Abstract
We have discovered a soluble, high affinity, specific zeatin-binding activity that correlates with hypersensitivity to cytokinin in embryogenic embryoids of oilseed rape, evidence that the protein responsible is a cytokinin action receptor. We shall purify the zeatin-binding protein to homogeneity by FPLC and affinity chromatography, raise antibodies, develop a sensitive ELISA and so determine receptor abundance at different stages in development. Receptor distribution will be mapped by immunohistochemistry. cDNA to mRNA from tissue rich in receptor will be enriched in receptor sequence by subtractive hybridisation using mRNA from control tissue (with undetectable receptor activity) and the receptor sequence identified using a synthetic probe based on amino acid sequence data from the purified receptor protein. The nucleic acid sequence of the receptor will enable a full molecular biology study to be initiated.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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