BBSRC Portfolio Analyser
Award details
Cloning and characterisation of signalling proteins downstream of the auxin binding protein
Reference
C01225
Principal Investigator / Supervisor
Professor Paul Anthony Millner
Co-Investigators /
Co-Supervisors
Institution
University of Leeds
Department
Inst of Molecular & Cellular Biology
Funding type
Research
Value (£)
167,239
Status
Completed
Type
Research Grant
Start date
21/01/1994
End date
31/10/1997
Duration
45 months
Abstract
Synthetic peptides corresponding to the C-terminus of Zea auxin binding protein (ABPzml) will be used for identification and affinity isolation of a transmembrane docking protein (ABP-DP1) which will be cloned and expressed in the Baculovirus/sf9 cell system. Interaction of ABPzml and ABP-DP1 will be characterised and downstream elements sought via affinity isolation and contingent replication assay. Signalling events downstream of ABPzml will be probed in vivo via electrophysiological and ion imaging studies on Vicia and Zea guard cells challenged with ABPzml C-terminal peptides.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search