BBSRC Portfolio Analyser
Award details
Molecular cloning, heterologous expression and characterisation of gibberellin 20-oxidases
Reference
C01207
Principal Investigator / Supervisor
Professor Chris Willis
Co-Investigators /
Co-Supervisors
Institution
University of Bristol
Department
Chemistry
Funding type
Research
Value (£)
122,113
Status
Completed
Type
Research Grant
Start date
28/02/1994
End date
28/07/1997
Duration
41 months
Abstract
A cDNA clone for the gibberellin 20-oxidase from Cucurbita maxima cotyledons will be used to isolate homologous clones from Pisum sativum cotyledons. cDNA from both sources will be expressed in Escherichia coli to produce recombinant enzymes for structure and mechanism studies. Isotopically-labelled gibberellin analogues will be used in combination with high-field NMR to study the mechanism of the loss of C-20 in gibberellin biosynthesis. Affinity reagents will be prepared as probes in mapping the enzyme active centre. The importance of individual amino acids at the active site will be determined by site-directed mutagenesis.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search