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Polarising gradients of calcium and calmodulin which specify cell diversity

Reference	C01059
Principal Investigator / Supervisor	Professor Tony Trewavas
Co-Investigators / Co-Supervisors	Professor David Cove, Professor Nick Read
Institution	University of Edinburgh
Department	Inst of Cell, Animal and Population Biol
Funding type	Research
Value (£)	131,108
Status	Completed
Type	Research Grant
Start date	01/04/1994
End date	01/04/1997
Duration	36 months

Abstract

Polarised cell divisions usually precede critical changes in cell specification. We will investigate the postulated role of intracellular gradients of calcium and calmodulin in polarising Physcomitrella protoplasts by (1) visualising gradients of calcium and calmodulin in living protoplasts by fluorescence ratio imaging and photometry and by using caged calcium and IP3 and a microbeam of UV light to artificially construct calcium gradients in living protoplasts (2) assess the significance of calmodulin gradients using specific peptides whose sequence represents the calmodulin binding domains of myosin light chain kinase, MARCKS, a growing calmodulin/actin bundling protein and neuromodulin which localises calmodulin in the growing region of cells. (3) transforming protoplasts with constructs containing both sense and antisense calmodulin.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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