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Molecular biology of an over-expressed Neurospora enzyme: the basis of allostery and intra-allelic complementation in glutamate dehydrogenase

Reference	C01034
Principal Investigator / Supervisor	Professor David Rice
Co-Investigators / Co-Supervisors	Professor Geoffrey Turner
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	308,794
Status	Completed
Type	Research Grant
Start date	01/10/1993
End date	01/05/1997
Duration	43 months

Abstract

The NADP+-dependent glutamate dehydrogenase (GDH) gene of Neurospora crassa has been cloned and over-expressed at Reading. A homologous GDH structure has been solved at Sheffield. N. crassa GDH shows marked allosteric behaviour and also intra-allelic complementation between defective mutants, mediated by the oligomeric structure. We aim to explain these properties by modelling, X-ray crystallography and functional studies (enzymology), using IFR's bank of cloned and sequenced GDH mutants. Hypotheses will be tested by site-directed mutagenesis.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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