Award details

Investigating the properties of prion proteins using modelling linked to protein characterisation

Reference	BSB11948
Principal Investigator / Supervisor	Dr James Warwicker
Co-Investigators / Co-Supervisors	Dr James Hope
Institution	The University of Manchester
Department	Life Sciences
Funding type	Research
Value (£)	71,986
Status	Completed
Type	Research Grant
Start date	01/01/2000
End date	14/04/2003
Duration	40 months

Abstract

Solution structures of the PrP-sen folding core and characterisations of recombinant PrP folding and unfolding are available, but definitive links to the mechanism of PrP-sen/PrP-res conversion have not been established. Insolubility (PrP-res) and flexibility (PrP-sen) complicate further structure determination. The proposal links PrP modelling expertise with biophysical studies and in vitro conversions, to probe the folding and interactions of wild- type and mutant PrPs. For example, unfolding of mutants PrPs will test the predicted basis for beta-rich intermediate formation, whilst the conversion reaction will be used with mutants, pH-variation, and under varying conditions, to develop models for PrP- PrP interaction.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Biology of Spongiform Encephalopathies - BMS Com (BSEBMS) [1999]
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search