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De novo induction of a yeast prion
Reference
BS516364
Principal Investigator / Supervisor
Professor Emeritus Mick Tuite
Co-Investigators /
Co-Supervisors
Institution
University of Kent
Department
University of Kent Department
Funding type
Research
Value (£)
263,076
Status
Completed
Type
Research Grant
Start date
06/06/2002
End date
06/06/2005
Duration
36 months
Abstract
In this project, we will explore the underlying molecular and genetic events associated with de novo prion formation in the yeast Saccharomyces cerevisiae. Using the Sup35p/[PSI+] prion as a model, we will isolate and characterise strains in which the [PSI+] prion has arisen de novo and use molecular genetic and biochemical techniques to identify the underlying molecular trigger. The importance of Sup35p oligopeptide repeats as well as cell physiology and ploidy in influencing the rate of de novo conversion will be assessed. Finally, affinity-tagged Sup35p will be used to analyse the Sup35p complexes that form de novo in the absence of overexpression of Sup35p.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Biology of Spongiform Encephalopathies - Phase 5 (BS5) [2001]
Funding Scheme
X – not Funded via a specific Funding Scheme
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